Fig. 3: Microsyntenty analysis of LS and CYP80F1 regions.

a Microsyntenty analysis of LS genes across 17 selected species. Rectangles represent annotated genes with orientation on the reverse strand (green) and same strand (blue). LS gene names from A. tanguticus, B. arborea, and M. caulescens were marked above the gene blocks. The lines associating the syntenic LS genes are highlighted in red. The gray lines indicate the gene collinearity across candidate species. b The position of LS residues of Iochroma cyaneum with black rounded rectangles indicating exons. c Biosynthetic pathway from the littorine and hyoscyamine aldehyde branch. d Syntenic assessment of CYP80F1 genes between P. inflata, Pepper, B. arborea, A. tanguticus, and M. caulescens. CYP80F1 gene names from A. tanguticus, B. arborea, and M. caulescens were indicated above the gene blocks. The black dotted rectangle indicates the position of CYP80F1 residues, while the green rounded rectangles in the dashed box indicate exons. The lines indicate genes having collinearity, and pink lines indicate colinear relationships of CYP80F1 genes between P. inflata, B. arborea, A. tanguticus, and M. caulescens. The pink dashed lines signify the presumed collinearity of incomplete CYP80F1 genes (pseudogene) across three Capsicum species.