Fig. 6: Characterized enzymatic activities of PPAR, CYP80F1, HDH, and H6H. | Nature Communications

Fig. 6: Characterized enzymatic activities of PPAR, CYP80F1, HDH, and H6H.

From: Multiple independent losses of the biosynthetic pathway for two tropane alkaloids in the Solanaceae family

Fig. 6

a Extracted-ion chromatograms (EIC) illustrating the in vitro activity of from BaPPAR, McPPAR and PiPPAR with phenylpyruvic acid used as substrate. b Extracted-ion chromatograms showing the in vivo activity of four CYP80F1 genes from A. tanguticus (AtCYP80F1), B. arborea (BaCYP80F1), M. caulescens (McCYP80F1) and A. belladonna (AbCYP80F1) via coexpressing of AbHDH, AtHDH, BaHDH, McHDH, ItHDH (I. triloba), and InHDH (I. nil) genes, for converting littorine into hyoscyamine in tobacco leaves. Littorine was used as substrates by co-infiltration into tobacco leaves. c Extracted-ion chromatograms (EIC) illustrating the in vitro activity of McH6H and LcH6H with hyoscyamine or anisodamine used as substrate. Source data are provided as a Source Data file.

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