Table 1 Features of genome assemblies and annotations
Category | A. tanguticus | L. chinense | B. arborea | M. caulescens |
---|---|---|---|---|
Sequencing | ||||
Platform | Nanopore | PacBio | PacBio | PacBio |
Genome-sequencing depth (X) | 105.77 | 35.57 (valid) | 20.09 (valid) | 33 (valid) |
Assembly | ||||
Estimated genome size (Mb) | 1198 | 1408 | 1517 | 756 |
Assembled genome size (Mb) | 1249 | 1538 | 1548 | 712 |
N50 of scaffolds (bp) | 49,959,515 | 132,783,878 | 121,782,173 | 28,080,016 |
No. of contigs | 205 | 1,406 | 1,398 | 808 |
N50 of contigs (bp) | 23,808,256 | 2,994,494 | 7,701,694 | 25,262,060 |
GC content of the genome (%) | 37.05 | 37.66 | 35.22 | 35.18 |
Anchored to chromosome (%) | 97.47 | 98.53 | 97.40 | 94.67 |
Complete BUSCOs (%) | 98.10 | 94.90 | 98.10 | 98.30 |
Annotation | ||||
Percentage of repeat sequences (%) | 65.68 | 70.22 | 79.0 | 70.11 |
LTR rate (%) | 43.07 | 44.48 | 58.37 | 36.62 |
No. of predicted protein-coding genes | 46,606 | 54,946 | 32,347 | 29,193 |
Average gene length (bp) | 4846.05 | 3775.38 | 3880.88 | 5447.13 |
Average CDS length (bp) | 1134.68 | 1017.84 | 1128.02 | 1282.79 |
Mean exon/intron length (bp) | 216.06/872.93 | 236.46/834.45 | 230.10/705.45 | 222.87/875.65 |
Mean exon number per gene | 5.25 | 4.30 | 4.90 | 5.76 |