Fig. 5: Possible histidine labeling traces of FUI and FUII strains. | Nature Communications

Fig. 5: Possible histidine labeling traces of FUI and FUII strains.

From: Engineering a synthetic energy-efficient formaldehyde assimilation cycle in Escherichia coli

Fig. 5

From 13C-labeled formaldehyde and unlabeled DHAP, fructose-1,6-bisphosphatase activity from YggF, YbhA, GmhB, or PhoA generates unlabeled F6P, resulting in histidine (His) unlabeled and once labeled (a). Histidine would be in unlabeled and once labeled when EuMP operates via the sedoheptulose 1,7-bisphosphatase (SBP) route (b). And it would be twice 13C-labeled when EuMP operates in the transaldolase (TAL) manner (c).

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