Fig. 4: IL-17A activates the BCR-ABL and JAK/STAT3 signaling pathways to promote the proliferation of Ph⁺ B-ALL cells.

a Volcano plot of differentially expressed genes in primary mouse B-ALL cells with or without rmIL-17A treatment. According to the criteria of Log2(fold-change) >1.5 or <-1.5 and P value < 0.05, selected upregulated and downregulated genes are highlighted in the volcano plot. b Molecular signature classification screening by GSEA with the top enriched signaling pathways in primary mouse leukemia cells treated with or without rmIL-17A. a, b n = 3 independent experiments. c GSEA demonstrating the enrichment of gene sets in Ph⁺ B-ALL patients with IL-17RA mRNA ⁽205707_at) expression above the median level (IL-17RA high) (n = 61) and below the median level (IL-17RA low) (n = 61). d GSEA demonstrating the enrichment of indicated gene set in B cells from Ph⁺ B-ALL patients and HDs in GSE134759 dataset. e Real-time PCR analysis of the relative mRNA levels of Jak2 and Il-6 in primary mouse leukemia cells treated with or without rmIL-17A (n = 3 independent experiments). f, g The human IL-6 concentrations in the culture supernatant of SupB15 cells (n = 3 independent experiments) (f) and primary Ph⁺ B-ALL cells (g) treated with different concentrations of rhIL-17A were measured by ELISA (n = 4 independent experiments). h Real-time PCR analysis of the relative mRNA levels of Jak2 and Il-6 in primary mouse leukemia cells treated with pathological concentrations of rmIL-17A (n = 3 independent experiments). i The levels of proteins in primary mouse leukemia cells treated with or without rmIL-17A were measured by Western blotting. n = 3 independent experiments. The samples were derived from the same experiment, and the gels/blots were processed in parallel. a–d Statistical significance was determined by a one-sided permutation test, and statistical adjustments were made for multiple comparisons. Statistical significance was calculated by (e, g) two-tailed Student’s t-test; (f, h, i) one-way ANOVA with Tukey’s multiple comparison tests; Data are presented as means ± S.E.M. Source data are provided as a Source Data file.