Fig. 2: GliT:GFP and GtmA:GFP localize in the cytoplasm, endoplasmic reticulum, and small vesicle structures during GT self-protection. | Nature Communications

Fig. 2: GliT:GFP and GtmA:GFP localize in the cytoplasm, endoplasmic reticulum, and small vesicle structures during GT self-protection.

From: Aspergillus fumigatus mitogen-activated protein kinase MpkA is involved in gliotoxin production and self-protection

Fig. 2

GliT:GFP and GtmA:GFP germlings were grown in liquid MM for 24 h at 37 oC and exposed to GT 3 µg/ml for 2 h and co-stained with either CMAC, ER-Tracker Blue-White DPX, or FM4-64 a The number of GliT:GFP and GtmA:GFP germlings that co-localized with CMAC, ER-tracker, or FM4-64 staining were determined. We have counted two independent experiments with 45 germlings per experiment (N = 90 germlings) for each strain and the results were expressed as mean values (%) of the 2 independent experiments of GFP germlings that co-localizes with vacuoles (CMAC), Endoplasmic Reticulum, ER (ER-tracker) or vesicles (FM4-64). The percentage of germlings with punctuated structures of GliT:GFP and GtmA:GFP were also determined. Yellow arrows indicate the localization of GliT:GFP and GtmA:GFP in magnified images of sub-cellular structures. Germlings were assigned as positively present in the vacuoles, endoplasmic reticulum, vesicles, or endomembranes if at least one of these structures was co-localizing with GliT:GFP or GtmA:GFP in the same germling. Representative images are shown in (b), (c), (d), (e), (f), and (g). Bars, 5 µm. Source data are provided as a Source Data file.

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