Fig. 3: Identification of proteins that interact with GliT:GFP and GtmA:GFP during GT production. | Nature Communications

Fig. 3: Identification of proteins that interact with GliT:GFP and GtmA:GFP during GT production.

From: Aspergillus fumigatus mitogen-activated protein kinase MpkA is involved in gliotoxin production and self-protection

Fig. 3

A. fumigatus was grown for 24, 48, and 72 h at 37 oC in liquid Czapek-dox medium. Proteins were extracted and immunoprecipitated (IP) with anti-GFP antibody. a Venn diagram showing the number of unique and shared IP proteins identified by mass spectrometry as interacting with GliT:GFP and/or GtmA:GFP. b–d Fungifun categorization of proteins interacting with GliT:GFP, GtmA:GFP, and GliT:GFP and GtmA:GFP. (e and f) Co-IPs for GliT:GFP, GtmA:GFP, GliT:GFP MpkA:3xHA, GtmA:GFP MpkA:3xHA and MpkA:3xHA strains under GT production and self-protection. For GT production, A. fumigatus was grown under the same conditions described in (a) while for GT self-protection A. fumigatus was grown in liquid minimal medium for 24 h at 37 oC and exposed or not to GT 5 µg/mL for 30 min, 1 h and 3 h. The western blots are representative results from 2 independent experiments. Source data are provided as a Source Data file.

Back to article page