Fig. 6: Peroxisomes are required for GT production, self-protection, and virulence.

a The A. fumigatus wild-type, ΔpexE, and ΔpexG strains were grown for 5 days at 37 ^oC on MM and MM+biotin. b, c The A. fumigatus wild-type, ΔpexE, and ΔpexG strains were grown for 2 days at 37 ^oC on MM and MM + 30 µg/ml of GT. The radial growth results are the mean of 3 independent experiments ± SD, n = 6 biological replicates (*, p < 0.05, t-test) d The A. nidulans wild-type, ΔpexE, and ΔpexG strains were grown for 5 days at 37 ^oC on MM and MM+biotin. e, f The A. nidulans wild-type, ΔpexE, and ΔpexG strains were grown for 2 days at 37^oC on YUU and YUU + 5 µg/ml of GT. Results are the mean of 3 independent experiments ± SD, n = 6 biological replicates (*, p < 0.05, t-test). g, h GT and bmGT production in the A. fumigatus wild-type, ΔpexE, and ΔpexG strains. The strains were grown in liquid Czapek-dox medium for 3 days at 37 ^oC. GT and bmGT were extracted from the supernatants and analyzed by mass spectrometry. Results are the mean of n = 6 biological replicates from 3 independent experiments ± SD; (*, p < 0.05, t-test). i, j Kaplan-Meier survival curves showing percentages of immunosuppressed mice infected intranasally with A. fumigatus strain (n = 10 mice/strain) and percentage of variation in the body weight. Phosphate buffered saline (PBS) was administered in a negative control group (n = 10). The indicated p-values determined with the use of the Logrank (Mantel-Cox test) and Gehan-Breslow-Wilcoxon test comparing the ΔpexE, and ΔpexG mutants with the wild-type strain, (*, p < 0.05 and **, p < 0.001). In the percentage of body weight variation, ΔpexE mutants are compared to the wild-type while ΔpexG mutants are compared to the PBS negative control (*, p < 0.001, One-way ANOVA with Tukey’s multiple comparisons test). Data represent the cumulative data of 2 separate experiments. Source data are provided as a Source Data file.