Fig. 2: Phenotype analysis of Styxl1 knockout male mice.
From: STYXL1 regulates CCT complex assembly and flagellar tubulin folding in sperm formation
A Schematic diagram of targeting strategy by CRISPR/Cas9 and the knockout alleles in the two founder lines. B Quantification of relative expression levels of STYXL1 protein in adult Styxl1Δ74bp/Δ74bp and Styxl1Δ4bp/Δ4bp testes by PRM (n = 3 mice per group). ***p < 0.0001 using one-way ANOVA followed by Dunnett’s multiple comparisons test. C Litter size of adult Styxl1-/- male and Styxl1-/- female mice (n = 5 mice for Styxl1+/+ female group, n = 4 mice for Styxl1-/- female group). NS p = 0.9977 and ***p < 0.0001 using one-way ANOVA followed by Dunnett’s multiple comparisons test. D Statistics analysis of adult Styxl1+/+ and Styxl1-/- testis/body weight ratio (n = 3 mice per group). NS p = 0.1670 using two-tailed Student’s t-test. E–G Quantitative analysis of sperm count (**p = 0.0020 using two-tailed Student’s t-test) (E), sperm motility (***p = 0.0002 using two-tailed Student’s t-test) (F) and progressive motility (***p = 0.0006 using two-tailed Student’s t-test) (G) of adult Styxl1+/+ and Styxl1-/- mice (n = 4 mice per group). H H&E-stained caput epididymis and cauda epididymis of Styxl1+/+ and Styxl1-/- mice. Scale bar:50μm. I, J The morphologies of Styxl1-/- sperm by H&E staining (I) and the percentage of sperm abnormalities (**p = 0.0046 using two-tailed Student’s t-test) (J) in Styxl1-/- cauda epididymis compared with controls (n = 3 mice per group). Scale bar:5μm. NS, not significant; **, p < 0.01; ***, p < 0.001. Data are presented with the mean ± SEM. n = 3 biologically independent samples were included in each group (H, I). Source data are provided as a Source Data file.
