Fig. 4: Dysregulation of flagellar proteins in Styxl1-/- sperm.
From: STYXL1 regulates CCT complex assembly and flagellar tubulin folding in sperm formation
A Colocalization analysis of HA-STYXL1 (yellow) and AC-TUBULIN (red) in primary cilia of HA-STYXL1 overexpressed NIH3T3 cells after serum starvation. Scale bar: 5μm. B, C Immunofluorescence analysis (B) and length statistics (C) of primary cilia in NIH3T3 cells treated by Styxl1 shRNA with or without HA-STYXL1syn overexpression after serum starvation (n = 87 cells examined for Scramble group, n = 73 cells examined for shStyxl1 2# group, n = 106 cells examined for shStyxl1 3# group, n = 92 cells examined for shStyxl1 2# + HA-STYXL1syn group, n = 86 cells examined for shStyxl1 3# + HA-STYXL1syn group over three independent experiments). ***p < 0.0001 using one-way ANOVA followed by Dunnett’s multiple comparisons test. AC-TUBULIN (red) and DAPI (blue) were used to stain cilia and nuclei, respectively. Scale bar: 5μm. D Heatmap of differentially expressed proteins between Styxl1+/+ and Styxl1-/- sperm. Enriched terms of biological processes (E) and cellular components (F) are shown. ***, p < 0.001. Data are presented with the mean ± SD. Source data are provided as a Source Data file.
