Fig. 5: The interactions of CCT complex subunits with STYXL1.
From: STYXL1 regulates CCT complex assembly and flagellar tubulin folding in sperm formation
A The list of CCT complex subunits identified by MS in GST pull-down assay of STYXL1. B Yeast two-hybrid assay (Y2H) for validating interactions between STYXL1 and CCT complex subunits. SUN1C and KASH5 were used as positive controls, and empty vectors of PGBKT7 (-) and PBAKT7 (-) were used as negative controls. C Schematic representation of full-length (FL), N-terminal (N, 1-167 aa), C-terminal (C, 168-310 aa) of STYXL1. D co-IP assay of interactions between full length, N-terminal or C-terminal regions of STYXL1 and CCT1, CCT6 or CCT7. E The relative interaction levels between different regions of STYXL1 and CCT1, CCT6 or CCT7 normalized by input levels. n = 3 biologically independent samples were included in each group (A, B).
