Fig. 6: The expression and function of CCT complex subunits.
From: STYXL1 regulates CCT complex assembly and flagellar tubulin folding in sperm formation
A Western blot analysis of CCT1, CCT6 and CCT7 in Triton-soluble, SDS-soluble and SDS-resistant fractions of mouse sperm. B Immunofluorescence analysis of AC-TUBULIN (green) and CCT1, CCT6 or CCT7 (yellow) in Styxl1+/+ and Styxl1-/- sperm with nuclei stained by DAPI (blue). Scale bar: 10μm. C Western blot and quantitative analysis of CCT1, CCT6 and CCT7 in sperm lysate from Styxl1+/+ and Styxl1-/- mice (n = 3 mice per group). PRM2 was used as a loading control. ***p = 0.0010 for CCT1, NS p = 0.5249 for CCT6, NS p = 0.9908 for CCT7 using two-tailed Student’s t-test. D, E Primary cilia stained with AC-TUBULIN (red) and DAPI (blue) after serum starvation in NIH3T3 cells treated with Cct1 siRNAs with or without FLAG-CCT1syn overexpression (D) and quantitative analysis of cilia length (E) (n = 59 cells examined for Scramble group, n = 57 cells examined for siCct1 1# group, n = 86 cells examined for siCct1 3# group, n = 37 cells examined for siCct1 1# + FLAG-CCT1syn group, n = 48 cells examined for siCct1 2# + FLAG-CCT1syn group over three independent experiments). *p = 0.0400 for Scramble vs siCct1 1#, *p = 0.0382 for siCct1 1# vs siCct1 1# + FLAG-CCT1syn, ***p < 0.0001 for Scramble vs siCct1 3#, ***p < 0.0001 for siCct1 3# vs siCct1 3# + FLAG-CCT1syn using one-way ANOVA followed by Dunnett’s multiple comparisons test. Scale bar: 5 μm. NS, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001. Data are presented as mean ± SD. n = 3 biologically independent samples were included in each group (A, B). Source data are provided as a Source Data file.
