Fig. 7: Abnormalities of CCT complex assembly and tubulin polymerization after Styxl1 deletion.
From: STYXL1 regulates CCT complex assembly and flagellar tubulin folding in sperm formation
A Gel filtration analysis of CCT complex proteins and CCT substrate proteins α-TUBULIN and β-TUBULIN in scramble or Stxyl1 shRNA treated NIH3T3 cells. n = 3 biologically independent samples were included. (B, C) Western blots of α-TUBULIN and β-TUBULIN in the total lysate (α-TUBULIN: NS p = 0.2634 for Scramble vs shStyxl1 2#, NS p = 0.4886 for Scramble vs shStyxl1 3#; β-TUBULIN: NS p = 0.9977 for Scramble vs shStyxl1 2#, NS p = 0.9546 for Scramble vs shStyxl1 3# using one-way ANOVA followed by Dunnett’s multiple comparisons test) B and pellet fractions (α-TUBULIN: **p = 0.0070 for Scramble vs shStyxl1 2#, *p = 0.0182 for Scramble vs shStyxl1 3#; β-TUBULIN: *p = 0.0101 for Scramble vs shStyxl1 2#, *p = 0.0239 for Scramble vs shStyxl1 3# using one-way ANOVA followed by Dunnett’s multiple comparisons test) C of scramble or Stxyl1 shRNA treated NIH3T3 cells in the MT sedimentation assay, and quantification of tubulin levels in corresponding fractions (n = 3 biological replicates per group). Data were presented with the mean ± SEM. D, E Western blots of α-TUBULIN and β-TUBULIN in WT and Styxl1-/- sperm with PRM2 as a loading control (n = 3 mice per group). **p = 0.0047 for α-TUBULIN, *p = 0.0229 for β-TUBULIN) using two-tailed Student’s t-test. Data are presented with the mean ± SD. F, G Western blot and quantification analysis of α-TUBULIN (F) (*p = 0.0240 and ***p < 0.0001 using two-tailed Student’s t-test) and β-TUBULIN (G) (*p = 0.0205 for ratio Lysate/PRM2, *p = 0.0241 for ratio MT pellet/Lysate using two-tailed Student’s t-test) in the total lysate and pellet in the MT sedimentation assay of Styxl1+/+ and Styxl1-/- sperm (n = 3 mice per group). Data were presented with the mean ± SEM. NS, not significant, *, p < 0.05, **, p < 0.01, ***, p < 0.001. Source data are provided as a Source Data file.
