Fig. 7: Antioxidant capacity of wildtype and ~ 3K region reversed ST11 hv-CRKP.

Antioxidant capacity of ST11 hv-CRKP C1789 were assessed by a ABTS and b SOD activities determination assays. Relative scavenging rates was the calculated clearance per concentration unit. The SOD activity assay was performed with six biological replicates. *p = 0.021, **p = 0.010, ****p = 2.01E-05. c The growth curves of strains in 5 mM H₂O₂. Growth differences were determined by comparing average growth rates over a two-hour period during the logarithmic growth phase (*p = 0.02). d The intracellular ROS of strains after stimulation with 1 mM H₂O₂. *p = 0.016, **p = 0.002. e The survival rates of C1789 and its reversed strains in macrophage. ****p < 0.001. f Utilizing laser confocal microscopy to visualize the survival of bacteria within macrophages. F-actin were stained by rhodamine phalloidin (green), and nuclei were counterstained with DAPI (blue). The bacteria were labeled with pHrodo (red). Scale bar = 5 µm. The representative result is present in f, and other images could be found in Supplementary Figs. 9 and 10. Two-tailed unpaired t-tests were used to compare C1789::pEasy vs. C1789::hp1-4/C1789::hp5 in a–e. The bars and error bars present mean with SD. Three biologically independent experiments were performed for assays in a, c–f. Source data are provided as a Source Data file.