Fig. 2: Loss of Sox9 compromises beta cell integrity.
From: Sox9 regulates alternative splicing and pancreatic beta cell function
A Immunostaining analysis of control or Ins-Cre;Sox9-/- tissue from animals between 9-12 months of age. Images are representative of at least three animals per group from three separate cohorts. Insulin, Glut2, and Proinsulin staining is shown. Size bar, 20 μm. B RNA-seq analysis of islets isolated from control or Ins-Cre;Sox9-/- animals (13-16 months old, n = 4 per group) revealed a significant change in 196 genes. C Heat map of upregulated (red) and downregulated (blue) genes from the RNA-seq differential gene expression analysis is shown. Expression values are represented as colors and range from red (high expression), pink (moderate), light blue (low) to dark blue (lowest expression). D Gene ontology and KEGG pathway analysis of the genes that are significantly changed in the Ins-Cre;Sox9-/- islets is shown. E, F Enrichr analysis to identify significant overlaps between genes changed in the Sox9 knockout islets and those downstream of other transcriptional regulators, including Sox9. G GSEA showing genes participating in the “Protein Secretion” and “Pancreas beta cell genes” gene sets. Heat maps of the genes populating these pathways are shown next to the corresponding pathway. Source data are provided as a Source Data file.
