Fig. 5: Disassociation of co-localization of collagen type IV from integrin β receptor.

a, b Confocal image overlays of collagen type IV (EMB-9::mCherry) in red and integrin β receptor (PAT-3::GFP) in the green of the head region (a) and midbody region (b) at day 1 and day 8 of adulthood. The yellow color indicates colocalization. Anterior to the left, ventral side down. Scale bar = 10 µm. c, d Quantification of total EMB-9::mCherry (c) and PAT-3::GFP (d) intensity levels. Individual dots represent animals. Mean + SEM. One-way ANOVA for the P value. 1 independent biological trial is shown. Raw data and statistics are in Supplementary Data 11. e Quantification of colocalization by correlation of per-pixel red (EMB-9::mCherry) and green (PAT-3::GFP) intensities of the midbody region (b), which declined during aging in wild type but was maintained upon daf-2(RNAi) longevity intervention. One-way ANOVA for the P value. a–e For individual pictures, raw data, and statistical analysis, see Supplementary Data 11. f Longevity intervention daf-2(RNAi) prolonged collagen expression (Pcol-144::GFP) of wild type (green line) during aging but not in a perlecan unc-52(e669, su250) mutant background (aquamarine line) at permissive temperature 15°C. The age-dependent unc-52(e669, su250) mutant paralysis phenotype (red line) was delayed by daf-2(RNAi) (dashed red line) at 15°C. For details, see Supplementary Data 12. g Continuing with these same animals, unc-52(e669, su250) mutants completely suppressed daf-2(RNAi) longevity at 15°C. For details, see Supplementary Data 7.