Fig. 2: The primary structure of FAM161A is under selective pressure in rodents.

a Calculated extended identity ratios. Hs Homo sapiens, Mm Mus musculus, Bt Bos taurus, Oc Oryctolagus cuniculus. b Phylogenetic tree showing the evolutionary position of the four mammals used in the extended identity ratio calculations and their proximal (PC) and distal (DC) centriolar structures⁸². c The top 10 identity ratio (IR) hits and their extended identity ratios (EIR). Extended identity ratios were calculated for proteins near the FAM161A genomic location (d), proteins influenced by sperm competition (e), and sperm distal centriolar proteins (f). Rodent phylogenetic tree (g) with FAM161A sequence identity relative to human FAM161A (h). Percent identity is also shown as average ± SD for individual clades. *P < 0.05, **P < 0.01 (unpaired, two-tailed t test; exact p-values are provided in the figure and Source Data File); ns not significant. (i) Bayesian phylogeny of FAM161A inferred using nucleotide sequences of mammalian species. (j) Bayesian phylogeny of FAM161A inferred using nucleotide sequences of Myomorpha species. The scale bars in i and j represent the number of nucleotide substitutions per site.