Fig. 4: Syt1 droplets fuse with each other, and recover after photobleaching.
a Time series showing two droplets of syt1 C2AB (80–421)-GFP (3 µM protein, 3% PEG 8000) fuse and relax into a larger droplet. b Photobleaching a syt1 C2AB (80–421)-GFP droplet and subsequent fluorescence recovery, shown in a representative time series. (See Supplementary Movies 4, 5 for the movies) c Quantification of the FRAP experiments from n = 6 independent experiments, each containing 6–10 bleached droplets (total n = 47). The data were fitted with a hyperbolic function using Graphpad Prism (solid line); error bars represent SEM. The inset shows the t1/2 of the fluorescence recovery. The droplet and bleached region diameters were 2–2.5 µm and 1.6 µm, respectively. Source data are provided as a Source Data file. (Scale bars, 2 µm).
