Fig. 3: CTP binding modulates the DNA-binding activity of VirB in low-stringency conditions. | Nature Communications

Fig. 3: CTP binding modulates the DNA-binding activity of VirB in low-stringency conditions.

From: The virulence regulator VirB from Shigella flexneri uses a CTP-dependent switch mechanism to activate gene expression

Fig. 3

a Schematic of the biolayer interferometry (BLI) setup used for the analyses in panels b and c. A double-biotinylated dsDNA fragment (215 bp) containing a central virS sequence (in orange) was immobilized on a streptavidin-coated biosensor and probed with VirB (green). b, c BLI analysis of the DNA-binding behavior of VirB in the a absence and b presence of CTP (1 mM) in low-stringency buffer (150 mM NaCl). Biosensors carrying the target DNA (at a density corresponding to a wavelength shift of ~1.3 nm) were probed with the indicated concentrations of VirB. At the end of the association reactions, the biosensors were transferred into protein- and nucleotide-free buffer to monitor the dissociation reactions (wash). The graphs show the results of a representative experiment (n = 3 independent replicates).

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