Fig. 1: Photoactivation of OLPVR1 elicits CaCC currents in Xenopus oocytes.

a Responses to a 10-s pulse of light of decreasing intensity. Current records were taken every 50 s from the same oocyte injected with 30 ng OLPVR1 RNA clamped at +40 mV. Bath solution was ND96. b Average peak current, normalized to current at 100% intensity (75 µW mm^-2), vs. light intensity obtained with the protocol of a, applied to 4 oocytes (Error bars, SEM). c Photocurrents at different holding voltages from oocytes expressing OLPVR1 (30 ng RNA) or ChR2 (7.5 ng), in the specified different bath solutions. d OLPVR1 current-voltage relationships obtained from records as in c, in different extracellular ionic conditions. Control refers to currents recorded in non-injected oocytes. Currents were measured after 10-s illumination. (Error bars, SEM; n = 15, 11, 9, and 8 for 94 K⁺ 100 Cl^-, 94 Na⁺ 100 Cl^-, 94 K⁺ 10 Cl^-, and control, respectively). e OLPVR1 photocurrents recorded before (Black) and after (Red) 60’ incubation in ND96 solution containing 30 µM Ani9 or MONNA, inhibitors of TMEM16A CaCCs. Statistics are shown in Supplementary Fig. 1. f Photocurrents from OLPVR1-expressing oocytes (7.5 ng RNA) with and without intracellular injection of BAPTA (BAPTA_in) in ND96 bath solution. g Average peak photocurrent vs voltage in ND96 solution with and without injected BAPTA. (Error bars, SEM; n = 9 for ND96, n = 3 for +BAPTA_in). Source data are provided as a Source Data file.