Fig. 3: In mammalian cells, OLPVR1 localizes to the ER and activates surface Ca²⁺-activated channels through release of intracellular Ca²⁺.

a Confocal images of HEK293T cells cotransfected with plasmids for expression of the ER-marker DsRed2-ER (red) and GFP-fused OLPVR1 (green). Merge panel shows in yellow, overlapping ER (red) and OLPVR1 (green) signals. The nucleus is in blue and the plasma membrane in magenta. Image is representative of 18 cells from 3 independent transfections. b-d Whole-cell recordings were obtained from HEK293T cells transfected with OLPVR1 alone (b; n = 7) or with OLPVR1 and, either TMEM16A (c; n = 5) or SK1 (d; n = 8). Left panels: Current responses to illumination (green bars, 505 nm light). Cells were held at the indicated voltages. Right panels: Light-induced current (peak current elicited by first illumination – current before illumination) vs. voltage (Error bars, SEM). Currents were elicited by 400-ms voltage ramps from −100 to +100 mV repeated every second. The pipette solution had 10 µM EGTA, except for the current-voltage curve drawn in red (right panel of d) where it had 1 mM EGTA (n = 7). Source data are provided as a Source Data file.