Fig. 7: Pharmacologic inhibition of IGF2BP3 enhances anti-tumor activity of oHSV in glioma.

a, b Representative immunofluorescence images a and quantification b of NET formation in a co-culture system of GL261 cells and TANs. Scale bars, 20 μm. Representative images of n = 5. n = 5 biological independent samples. c The NETs-induced proliferation of GL261 cells in the co-culture system was detected by luciferase assay. n = 5 biological independent samples. d Electron microscopic analysis of TANs co-cultured with GL261 cells treated with the indicated agents. Yellow arrows indicate NETs. Scale bars, 5 μm. Representative images of n = 3. e Schedule of combination treatment for C57BL/6 mice subcutaneously injected with 1 × 106 GL261 cells. f, g Tumor weight and tumor survival were measured (n = 3 mice per group). h Representative images of xenograft tumors, “ND” indicates not detected. i Western blot analysis of protein expression levels of IGF2BP3, ICP0, ICP8, and gC. n = 3 biologically independent experiments. j Schedule of combination treatment for C57BL/6 mice intracranially injected with 1 × 10⁶ GL261 cells. k Kaplan-Meier analysis was performed on mouse models with intracranially implanted tumors derived from GL261 and treated with the indicated agents to assess survival (n = 3 mice per group). l Representative images of the luciferase signal from C57BL/6 mice inoculated with GL261 tumors and treated with the indicated agents. m Tumor size was estimated by quantifying luciferase activity in tumor cells (n = 3 mice per group). n HE staining analysis of tumor volume. The tumor size was indicated by a dashed line. o Immunohistochemistry analysis of IGF2BP3, ICP0, and neutrophil infiltration (CD66b, labeled with black arrows) in the indicated tumor tissues. Scale bars, 20 μm. p, q Representative immunofluorescence images p and quantification q of NET formation in the indicated tumor tissues. Scale bars, 20 μm. Representative images of n = 5. n = 5 biological independent samples. Statistical significance was determined using one-way ANOVA in b, c, f, m, q, or two-way ANOVA in g, or the log-rank (Mantel-Cox) test in k. Data represent the mean ± SD. Source data are provided in the Source Data file.