Fig. 4: Chemical modulation of PBS Piezo1 Variants by Yoda1. | Nature Communications

Fig. 4: Chemical modulation of PBS Piezo1 Variants by Yoda1.

From: PIEZO1 loss-of-function compound heterozygous mutations in the rare congenital human disorder Prune Belly Syndrome

Fig. 4

a Representative single-channel current recordings of WT Piezo1 and PBS variant proteins S260R, S2211L and co-expressed S260R + S2211L either without Yoda1 (Left) or with 20 µM Yoda1 in the pipette (Right). The data was obtained from cell-attached patches at −60 mV and −30 mmHg applied pressure. O and C denote open and closed state of the channel. b Overlay of the corresponding all-point current amplitude histograms obtained from (a) in the absence (blue) and presence of Yoda1 (dash-green line). c Steady state NPo of WT Piezo1 and variant proteins with and without Yoda1 calculated from (a) data. Experimental replica numbers for each condition are shown in the bar graph (n > 9). Analysis was performed using unpaired two-tailed t test, where ****p < 0.0001. Data points in panel c are represented as Mean ± SD.

Back to article page