Fig. 5: Yoda1 specifically stimulates Piezo1-dependent Calcium Influx in HEK293TΔP1 Cells expressing WT and PBS Piezo1 variants. | Nature Communications

Fig. 5: Yoda1 specifically stimulates Piezo1-dependent Calcium Influx in HEK293TΔP1 Cells expressing WT and PBS Piezo1 variants.

From: PIEZO1 loss-of-function compound heterozygous mutations in the rare congenital human disorder Prune Belly Syndrome

Fig. 5

Fluorescent detection of intracellular calcium influx via Fluo-4 dye with or without Yoda1 of WT and PBS Piezo1 variants channel activity. Ionophore A23187 (t = 24 min - red arrows) served as internal control to stimulate maximum non-specific calcium influx. a Addition of Yoda1 at 5 min (black arrow). Mock n = 8, WT n = 9, S260R n = 9, S2211L n = 15, Co-expressed n = 15. The insert shows the data from t = 12 min to t = 24 min. b Addition of DMSO at 5 min as a control (black arrow). Mock n = 4, WT n = 8, S260R n = 8, S2211L n = 12, Co-expressed n = 12. c Quantification of calcium influx indicated by normalized cell fluorescence induced by Yoda1. Data points reflect maximal activated signal prior to addition of ionophore A23187 (t = 24 min). Mock = empty vector transfection and RFU = Relative fluorescence units. Statistical analysis was performed by using One-way ANOVA where ***p < 0.001 and ns = not significant. Data points in (c) are represented as Mean ± SD.

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