Fig. 5: Circumferential motion of the actin nanoclusters is DAAM- and Zasp52-dependent. | Nature Communications

Fig. 5: Circumferential motion of the actin nanoclusters is DAAM- and Zasp52-dependent.

From: Emergence of periodic circumferential actin cables from the anisotropic fusion of actin nanoclusters during tubulogenesis

Fig. 5

a The Motion anisotropy analysis of small actin nanoclusters during circumferential cable formation at stage 15. The x axis corresponds to the longitudinal axis, whereas the y axis corresponds to the circumferential axis of the tracheal tube. The circumferentially biased motion indicated by the ratio (|∆y | /|∆x | ) larger than unity became apparent from −30 min. b Particle image velocimetry (PIV) analysis of the cortical actin dynamics in tracheal cells. The velocity vectors color-coded with the vector length are superposed. The minimum and maximum lengths (nm) of the vectors are indicated below the color box. Scale bar, 0.5 µm. c Transition of mean displacement (nm/s) of the nanoclusters from isotropic phase to anisotropic phase calculated by the velocity vectors of PIV analyses. Line plots represent mean ± SD. The two-sided Student’s t test was performed for the statistical analysis. P = 0.466 in −40 min (n = 4), P = 0.0729 in −30 min (n = 4), P = 0.0657 in −20 min (n = 3), P = 0.0176 in −10 min (n = 4), or P = 0.0038 in 0* min (n = 4). ∗P < 0.05, ∗∗P < 0.01. d The Motion anisotropy analysis of small actin nanoclusters in the genetically manipulated tracheal cells at early stage 16. e The PIV analysis of the cortical actin dynamics. Scale bar, 0.5 µm. f Comparison of mean displacement (nm/s) calculated via PIV analyses. Boxplots represent median plus minima and maxima with lower and upper quantiles. The two-sided Student’s t test was performed for the statistical analysis. P = 1.78e-6 in Control (n = 9), P = 0.728 in DAAM RNAi (n = 6), P = 0.436 in zasp52 RNAi (n = 10), or P = 8.98e-6 in myosin IIDN (n = 13). ∗P < 0.05, ∗∗P < 0.01. g Venn diagram showing the relationship between three factors that regulate actin patterning in silico: Nanocluster self-organization enabled by the interaction of actin filaments and crosslinkers (light yellow); Nanocluster fusion induced by myosin II contractility (light blue); and Anisotropy sensing (light red). The phenotypes of RNAi for nanocluster components are well categorized in the Venn diagram. See “Discussion” for details. Source data are provided as a Source Data file.

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