Fig. 2: Differential immune status in rodents versus humans might be determined by distinct macrophage responses against HTNV Infection.
From: Disparate macrophage responses are linked to infection outcome of Hantan virus in humans or rodents
a Natural infection phases of HTNV in A. agrarius mice defined based on the assessment of HTNV-S and anti-NP IgG in the lung tissue. b Cytokine production measured by ELISA in lung tissues (n = 20 in each group). HIES as control (vs. HIPS), p = 0.0092 (TNFα)/0.003 (IP-10)/0.5973 (MCP-1)/0.0275 (IFNα)/0.0084 (IL-1β)/0.0011 (IL-10). HINS as control (vs. HIES, HIPS or HICS), p = 0.0002/0.8384/0.9844 (TNFα); p = 0.0409/ > 0.9999/ = 0.4072 (IP-10); p = 0.0032/ 0.0203/0.937 (MCP-1); p < 0.0001/ = 0.0145/ = 0.4273 (IFNα); p = 0.9233/0.0069/0.1034 (IL-1β); p = 0.5576/ < 0.0001/ = 0.9844 (IL-10). c Representative immunoblot analysis of three independent experiments for p65 and Stat1 in AMs from field mice with various disease stages. d Representative immunoblot analysis of three independent experiments for p65 and Stat1 in mBMDM or hMDM from 0 to 36 hpi with an MOI of 1. (e and f) Representative immunoblot analysis of three independent experiments for TFs located in the cytoplasm or nucleus from mBMDM (E) or hMDM (F) at an MOI of 1 for HTNV infection. Data are shown as the mean ± SD for animal samples. Data are representative of three independent experiments. Analysis of different groups is performed with two-sided unpaired Student’s t test, or one-way ANOVA (Dunnett’s multiple comparisons test). *p < 0.05, **p < 0.01, ***p < 0.001; NS no significance. Molecular weight markers are shown to the left of the blots in kDa, and antibodies used are indicated to the right. Source data are provided as a Source Data file.
