Fig. 5: Notch pathway activates differently in human macrophages and promotes M1-mediated HFRS pathogenesis.

a qRT-PCR analysis of Notch pathway-related genes in HTNV-infected hMDM (MOI = 1, n = 4). Notch1, p = 0.0166 (0 vs. 24 hpi)/ < 0.001(0 vs. 48 hpi); Notch2, p < 0.0001 (0 vs. 48 hpi); Notch4, p < 0.0001 (0 vs. 24 or 48 hpi); Dll1, p < 0.0001 (0 vs. 24 or 48 hpi); Dll3, p < 0.001 (0 vs. 24 or 48 hpi); Jagged1, p < 0.0001 (0 vs. 48 hpi); Hes1, p = 0.0035(0 vs. 24 hpi)/ < 0.0001 (0 vs. 48 hpi); Hey1, p = 0.0004 (0 vs. 24 hpi)/ < 0.001 (0 vs. 24 hpi). b Representative immunoblot analysis of three independent experiments for the Notch pathway in HTNV-infected hMDM (MOI = 1). c Representative immunoblot analysis of three independent experiments for the NICD in the cytoplasm or nucleus in HTNV-infected hMDM (MOI = 1). d Representative immunofluorescent analysis of three independent experiments for NICD localization in HTNV-infected hMDM (MOI = 1). e Representative immunoblot analysis of three independent experiments for phosphorylated p65, ERK or JNK in HTNV-infected hMDM that are pretreated with DMSO or DAPT (50 μmol/L) for 12 h. f Supernatant cytokine concentration detected by ELISA from (e). DMSO vs. DAPT (48 hpi), p = 0.0012 (TNFα)/0.0099 (IL-1β)/0.0117 (IL-6)/0.0004 (MCP-1)/0.7738 (IFNα). qRT-PCR analysis of M1- (g) or M2-related (h) gene expression in hMDM from (e). In (g), p = 0.0048 (Tnf)/ 0.0112 (Il1b)/0.0028 (Nos2)/0.0019 (Ccl2); In (h), p = 0.0003 (Il10)/0.0028 (Tgfb)/0.003 (Agr1)/ < 0.0001 (Mrc1). Data are shown as the mean ± SEM, and are representative of three independent experiments. Analysis is performed using one-way ANOVA (a, Dunnett’s multiple comparisons test), or two-sided unpaired Student’s t test (f–h). *p < 0.05, **p < 0.01, ***p < 0.001; NS no significance. Molecular weight markers are shown to the left of the blots in kDa, and antibodies used are indicated to the right. Source data are provided as a Source Data file.