Fig. 3: Glucose transport and hexokinase are individually dispensable for C. parvum growth in vivo.

a Growth of CpGT1 tagging (TAG) or CpGT1 knockout (KO) transgenic strains b Growth of CpGT2 CpGT2 TAG or CpGT2 KO transgenic strains. NSG mice were infected with parasites and infection was monitored by measuring luciferase activity from fecal pellets collected at intervals post infection. Each line represents an individual NSG mouse (n = 6 for CpGT1 TAG, CpGT1 KO and CpGT2 TAG, n = 7 for CpGT2 KO, from two combined experiments). Two-way ANOVA corrected for multiple comparisons by Sidak’s method (*, P = 0.0250, ***, P = 0.0002 at 24 hpi, ***, P = 0.0001 at 30 hpi, ****, P < 0.0001). No significant difference in parasite burden was observed between CpGT1 TAG and CpGT1 KO strains. c Schematic representation of the reaction of hexokinase in the glycolytic pathway. d Diagram of the strategy to construct Cp hexokinase (HK) KO transgenic strain. e Growth of the CpHK KO transgenic strain. NSG mice were infected with parasites and infection was monitored by measuring luciferase activity from fecal pellets collected at intervals post infection. Each line represents an individual NSG mouse (n = 3 from one experiment). f PCR analysis of oocysts obtained from NSG mice infected with parasites as shown. Amplification products correspond to regions annotated in d. The experiment was performed twice with similar results. Source data are provided as an accompanying Source Data file.