Fig. 1: GABACeA neurons mediate acute restraint stress-induced anxiety-like behaviors.
From: Microglia govern the extinction of acute stress-induced anxiety-like behaviors in male mice
a Schematic of experimental design. Representative heatmaps (b) and summarized data of entries and the time spent in central area (c) of the open field test (OFT) from the indicated groups (0.5 h, n = 9 mice per group; 4 h, n = 9 mice per group; 8 h, n = 11 mice per group; 12 h, n = 8 mice per group). Representative heatmaps (d) and summarized data of entries and the time spent in the open arms (e) of the elevated plus maze (EPM) from the indicated groups (0.5 h, n = 9 mice per group; 4 h, n = 8 mice per group; 8 h, n = 11 mice per group; 12 h, n = 8 mice per group; e left, F1,64 = 23.89, p < 0.001; right, F1,64 = 42.23, p < 0.001). f Schematic of multi-channel electrode recordings. Raster plots and typical traces (g) and the quantitative data (h) of the spontaneous firings of GABACeA neurons from the indicated groups (n = 20 cells from six mice per group; h F1,152 = 43.48, p < 0.001). i Schematic of the experimental procedure. j Summarized data of entries and the time spent in the central area and open arms of OFT and EPM from the indicated groups (OFT: Control + mCherry, n = 7 mice, ARS-2h + mCherry, n = 8 mice, ARS-2h + hM4Di, n = 8 mice; left, F2,20 = 10.18, p = 0.0009; right, F2,20 = 10.68, p = 0.0007; EPM: Control + mCherry, n = 9 mice, ARS-2h + mCherry, n = 9 mice, ARS-2h + hM4Di, n = 7 mice; left, F2,22 = 6.391, p = 0.0065; right, F2,22 = 22.87, p < 0.001). Significance was assessed by two-way repeated-measures ANOVA with post hoc comparison between groups in (c, e, h), one-way ANOVA with post hoc comparison between groups in (j). All data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001; n.s., not significant. See also Supplementary Data 1. Source data are provided as a Source Data file.
