Fig. 9: FPRs expressed on neutrophils are necessary for the effect of SCIMP on the ALI model.
From: Exosomal secreted SCIMP regulates communication between macrophages and neutrophils in pneumonia
a The schematic workflow shows the study of the effect of murine SCIMPN on the ALI model by using WT or Fpr1/2-/- mice. b–g After transbronchially perfused with SCIMPN (50 μg/50 μL PBS per mouse mixed with 5 × 106 CFU E. coli, n = 5) or Vehicle (50 μL PBS per mouse mixed with 5 × 106 CFU E. coli, n = 5) for 4 h to WT or Fpr1/2-/- mice, neutrophils (Neu, CD11b+CD11c-Ly6G+) and alveolar macrophages (AM, CD11b+CD11c+F4/80+Ly6G-) in the BALF from the ALI model were measured by flow cytometry (b, c), and the bacterial count was measured by dilutional bacterial clone culture (d, e, n = 5), and the lung injury and inflammation were evaluated by histological examination of lung biopsies stained with H.E. (bar = 200 μm, f), and the survival of the four groups (n = 8 in each group) was observed and analyzed (g). The raw data is available in the “Source Data”.
