Fig. 6: Microbial inter-region relations along the GI tract.

A Bacterial prevalence in each organ by 16 S full-length sequencing. ASVs with relative abundance >0.1% (~10 sequencing reads) were considered as present on the organ. Red dot represents >50% prevalence. B The ratio of bacteria with positive correlations between each pair of organs among the prevalent bacteria (n = 76). The abundance of N. mucosa and F. nucleatum were plot in the right-side, with dash line links the same individual (P < 0.05, correlation analysis). Data are shown as Box and whisker plots to represent the median (center line), quartiles (box), range (whiskers), and outliers (points outside 1.5 times the interquartile range). Two-tailed Spearman correlation, Partial Spearman correlation, and two-tailed Pearson correlation were used simultaneously. C ASVs simultaneously present in the intra-individual upper GI (left) or intra-individual lower GI tract (right). Areas labeled in red represent the presence of ASV on all the organs from the same individual (relative abundance >0.1% for all). Light red color: one type of ASV of a particular species shared among organs from the same individuals. Dark red color: >1 ASVs of a particular species shared among organs from the same individuals. Bacterial prevalence in the oral cavity was displayed on left side of the plot. Source data are provided as a Source Data file.