Fig. 2: Morphology of MaSp2 droplets, nanofibrils and fibers as characterized by CLSM and SEM.

a N-R12-C labeled with DyLight-488 was transferred to the microfluidic device for spinning under native-like conditions. Three independent experiments were performed with similar results. Scale bar, 30 μm. b 3D reconstruction of N-R12-C droplets distributed along the channel. Scale bar, 5 μm. c Surface structure of N-R12-C droplets induced by LLPS inside the microfluidic device as visualized by SEM. Two independent experiments were performed with similar results. Scale bar, 10 μm. d N-R12-C fiber assembled inside the channel in section C3 with a hierarchical organization. Three independent experiments were performed with similar results. Scale bar, 10 μm. e 3D reconstruction of N-R12-C fiber aligned along the channel in section C3. Two independent experiments were performed. Scale bar, 5 μm. f Oriented nanofibrils observed from the flexible continuous fiber in Milli-Q water after recovering it from the microfluidic chip. Three independent experiments were performed with similar results. Scale bar, 10 μm. g Nanofibrils split from the fiber to show hierarchical organization upon exposure to the Milli-Q water. Three independent experiments were performed with similar results. Scale bar, 10 μm.