Fig. 6: Cas13d binding and nuclease activation follow distinct rules.
From: Massively parallel profiling of RNA-targeting CRISPR-Cas13d
Cas13d binding is penalized by distal RNA structures and mismatches. After initial distal recognition, the RNA duplex forms from the distal positions to the proximal positions. A mismatch in the proximal region fails to activate the nuclease activity, leading to a catalytically inactive enzyme. Matched target sequences that form a complete RNA duplex activate the nuclease activity.
