Fig. 5: In situ sweat Phe analysis for assessing serum levels and protein diet intake effects. | Nature Communications

Fig. 5: In situ sweat Phe analysis for assessing serum levels and protein diet intake effects.

From: Interindividual- and blood-correlated sweat phenylalanine multimodal analytical biochips for tracking exercise metabolism

Fig. 5

a Metabolic pathway of Phe along with serum and sweat Phe fluctuations caused by protein intake during exercise. b Dynamic changes of sweat Phe levels from two subjects with different BMI in three periods of exercise, including before and after protein diet intake as well as after rest. DPV data from 0.4 to 0.6 V. c Correlation between sweat AAs and Phe levels in the intake-exercise experiment from two different subjects. d Sensor-measured Phe concentrations in sweat samples versus corresponding ELISA readouts. Data was measured from all sweat samples collected in the above evaluation experiment. The solid line represents the linear-fitted trendline. e Comparative study of sweat and serum Phe levels in the three periods of exercise from subject #1 (top) and subject #5 (bottom). f Correlations between sweat and serum Phe levels before (top) and after (bottom) the sweat rate normalization from two different subjects. Lines represent the fitted trendlines. g, h Fluorescence microscopy images (g) and relative cell viability (h) of HaCaT cells after 2 or 4 days incubation in biocompatibility test (n = 2 independent experiments). Scale bar, 100 μm.

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