Fig. 4: VGLL1 interacts with TEAD4 to regulate human TELC induction from naive PSCs.

a Pie chart showing the distribution of VGLL1-binding sites throughout the annotated genomic locations in CUT&Tag of TELC-D5 cells differentiated from 4CL H9 ESCs. b Venn diagram showing the overlap between downregulated genes in shVGLL1 compared to shLuc control and genes bound by VGLL1 at promoter sites in TELC-D5 differentiated from 4CL H9 ESCs. c Enriched GO terms for genes bound by VGLL1 in TELC-D5 cells differentiated from 4CL H9 ESCs. P value was calculated using a hypergeometric test (one-sided) and adjusted for multiple testing using the Benjamini-Hochberg correction. d Motif analysis using HOMER showing the significantly enriched DNA-binding motifs at VGLL1-bound sites in TELC-D5 cells differentiated from 4CL H9 ESCs. P value was calculated using a hypergeometric test (one-sided). e Immunoprecipitation using lysates from TELC-D5 cells differentiated from 4CL H9 ESCs with anti-VGLL1 (left panel) or anti-TEAD4 (right panel) and subsequent Western blotting analysis with anti-TEAD4, anti-VGLL1, anti-YAP and anti-WWTR1. Representative of three independent experiments. f Pie chart showing the distribution of TEAD4-binding sites throughout the annotated genomic locations in TELC-D5 cells differentiated from 4CL H9 ESCs. g Pie chart showing the distribution of YAP-binding sites throughout the annotated genomic locations in TELC-D5 cells differentiated from 4CL H9 ESCs. h Venn diagram showing the overlap between VGLL1-, TEAD4- and YAP-bound sites in CUT&Tag of TELC-D5 cells differentiated from 4CL H9 ESCs. Representative cell cycle and TE genes corresponding to the overlapping regions are shown. i Co-occupancy analysis by signal density pileups of VGLL1, TEAD4 and YAP peaks in CUT&Tag of TELC-D5 differentiated from 4CL H9 ESCs. GO analysis for each group is shown. P value was calculated using a hypergeometric test (one-sided) and adjusted for multiple testing using the Benjamini-Hochberg correction.