Fig. 6: Conserved CCR7+ DC heterogeneity and CD8+ T cell crosstalk in human cancer. | Nature Communications

Fig. 6: Conserved CCR7+ DC heterogeneity and CD8+ T cell crosstalk in human cancer.

From: Tumour-retained activated CCR7+ dendritic cells are heterogeneous and regulate local anti-tumour cytolytic activity

Fig. 6

a UMAP of scRNA-seq of CCR7+ DCs from human CRC (GSE178341, n = 62 patients). b Selected gene expression in CCR7+ DCs from tumour (T), normal adjacent tissue (N) and tumour-dLN from independent scRNA-seq data of human CRC (syn26844071, n = 63 patients). c UMAP of myeloid cells from scRNA-seq of human metastatic urothelial carcinoma (mUC, n = 11 patients). d single-cell gene expression (scGEX) profiles associated with responders (complete responder, CR; partial responder, PR) or non-responders (stable disease, SD; progressive disease, PD) in the IMvigor 210 trial (atezolizumab) for treatment of mUC; Scissor integration of bulk RNA-seq of tumour samples from IMvigor210 (n = 208 patients) and scRNA-seq from c. e Differential gene expression in myeloid cells associated with responders (CR/PR) versus non-responders (SD/PD) from d. f Gene signature scores for Ccr7_DC.2 transcripts in scRNA-seq of CCR7+ DCs from human breast cancer following treatment with anti-PD-1 antibodies; T cell clonotype expanders (E, i.e. responders, n = 9 patients) versus non-expanders (NE, i.e. non-responders, n = 20 patients). g PICseq (myeloid-T cell doublets) of human NSCLC; expression of CCR7+ DC-T cell ligand-receptor pairs, grouped by myeloid cell identity in each myeloid-T cell doublet (n = 10 patients). h Gene signature scores for “GO:BP T cell mediated cytotoxicity (GO:0001913)” (HLA genes removed), in myeloid-CD8+ T cell PICs grouped by the myeloid cell identity. i, j Spatial correlation (Pearson R-value) of CCR7+ DC signature scores and selected CCR7+ DC-ligand receptors expressed by CD8+ T cells, in spatial transcriptomics (10X Genomics Visium) of independent human CRC tumour sections (n = 2). Two-sided Wilcoxon rank-sum test with Benjamini-Hochberg multiple-testing correction (b, e), or two-sided Wilcoxon rank-sum test (f, h) were used. Data are shown as box (median; box, 25th and 75th percentile; whiskers, 1.5*inter-quartile range) and violin plots (b, f, h).

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