Fig. 4: Identification of binding residues of Q-OB. | Nature Communications

Fig. 4: Identification of binding residues of Q-OB.

From: Early onset diagnosis in Alzheimer’s disease patients via amyloid-β oligomers-sensing probe in cerebrospinal fluid

Fig. 4

a Identification of Q-OB binding sites for Aβn–(n+5) fragments of a full-length Aβ1–42. Data of fluorescence values of Q-OB, and (b) the heatmap displays the spectrum of binding intensity on Aβn–(n+5) sequence (white to red, 0–100%) (λex/λem = 500/620 nm). Data points with its error range stands for mean s.d. derived from n = 2 independent experiments. Time-dependent fluorescence intensity of Q-OB with Aβ1–42 pre-treated molecular weight cut-off of filter, including (c) under 100 kilodaltons (kDa), (d) over 100 kDa (λex/λem = 490/620 nm) (mean \(\pm\) s.d., n = 3 independent experiments). e Time-dependent fluorescence intensity of ThT with Aβ1–42 (λex/λem = 450/480 nm) (mean \(\pm\) s.d., n = 3 independent experiments). The abbreviated words are FL, fluorescence; ThT, thioflavin T. Source data are provided as a Source Data file.

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