Fig. 5: In vitro and In vivo imaging of Alzheimer’s disease (AD) brain tissue sections.
a Fluorescence images of cortex area in the brain tissue slice from 16-month-old 5xFAD transgenic AD model mouse (upper panels) and hippocampal area of AD patients’ brain tissue (bottom panels) stained by Q-OB (1 μM, red and green channels) and 6E10 (anti-Aβ antibody, 1:200, blue channel) (red channel; λem/λex = 520/590 nm, green channel; λem/λex = 480/520 nm, and blue channel; λem/λex = 360/470 nm); Scale bar: 100 μm. b Pseudo-color overlapping of stained Aβ aggregates in human AD patient brain tissue marked by white-hollowed arrows (overlap of green and blue channels) and yellow arrows indicate autofluorescence; Scale bar: 100 μm. c Representative illustration of the blood-brain barrier (BBB) penetrability of Q-OB for the brain in vivo imaging. d Representative fluorescence signal of in vivo imaging and (e) average radiant efficiency, 50 min subsequent to tail vein injection of Q-OB to transgenic AD model mouse brains, including 13.5- and 3-month-old 5xFAD, and 2-month-old B6 wild-type mice. Error bars indicate s.d. (n = 3 biologically independent animals). The p-values were obtained with two-sided unpaired t-test; *p < 0.05, **p < 0.01. The exact p-values are provided in a Source Data file. The fluorescence images were captured with an IVIS imaging system at an excitation wavelength of 500 nm, and the emission was monitored at 620 nm. The abbreviated words are IV, intravenous; Tg, transgenic. Panel c was adapted from “Blood Brain Barrier (Transverse),” by BioRender.com (2023). Retrieved from https://app.biorender.com/biorender-templates. Source data underlying (e) are provided as a Source Data file.
