Fig. 4: Clustering analysis of the 41 unique NRA designs from K_trpD9923 reveals 5 distinct routes for overproducing anthranilate.
From: Rational strain design with minimal phenotype perturbation
Each design contains a unique set of enzymes to be targeted. SHKK Shikimate kinase, CHORS Chorismate synthase, DHQS 3-dehydroquinate synthase, CHORM Chorismate mutase, ANS Anthranilate synthetase, ANPRT Anthranilate phosphoribosyltransferase, DDPA 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase, GND Phosphogluconate dehydrogenase, PFK_3 Phosphofructokinase (s7p), RPI Ribose-5-phosphate isomerase, G6PDH2r Glucose 6-phosphate dehydrogenase, NADH5 NADH dehydrogenase, SUCDi Succinate dehydrogenase (irreversible), HEX1 Hexokinase, PGK Phosphoglycerate kinase, PYK Pyruvate kinase, PGM Phosphoglycerate mutase, PGI Glucose-6-phosphate isomerase, PPS Phosphoenolpyruvate synthase, GAPD Glyceraldehyde-3-phosphate dehydrogenase, FBA Fructose-bisphosphate aldolase, PFK Phophofructokinase, TPI Triose-phosphate isomerase, FBP Fructose-bisphosphatase, ENO Enolase, PSERT Phosphoserine transaminase, GLUDy Glutamate dehydrogenase, GLNS Glutamine synthetase, ACONTa Aconitase, AKGDH 2-Oxogluterate dehydrogenase, ICDHyr Isocitrate dehydrogenase, FUM Fumarate mutase, ICL Isocitrate lyase, ME2 Malic enzyme (NADP). Source data are provided as a Source Data file.
