Fig. 5: Performance of K_trpD9923 models implementing modifications of recombinant strains.
From: Rational strain design with minimal phenotype perturbation
a Simulated responses of W3110 trpD9923 (wt, orange), W3110 trpD9923/pJLaroGfbr (black) and W3110 trpD9923/pJLaroGfbrtktA (red) when they are implemented in K_trpD9923. For comparison, we provide the responses of the implemented NOMAD designs (blue). The bold lines and shaded regions represent the median and interquartile ranges of the responses across the 10 kinetic models. b Experimentally recorded data for the three strains. The solid circles and error bars represent the mean and standard deviations of the triplicate experiments. The models capture the experimentally observed trends, with the overexpression of tktA resulting in a superior anthranilate titer compared to the targeting of aroG alone. NOMAD designs provide a superior titer of anthranilate when compared with the in-silico implementation of the experimental designs. The temporal evolutions of extracellular glucose and biomass of the in-silico strains displayed in panel are provided in Supplementary Note 4. Source data are provided as a Source Data file.
