Fig. 7: Performance of NOMAD designs implemented in enhanced models.
From: Rational strain design with minimal phenotype perturbation
a Predicted temporal evolution of anthranilate titers of the NOMAD designs (blue) generated using enhanced models of the reference strain - eK_trpD9923. The solid lines and shaded regions denote the median and interquartile ranges, respectively. The designs attained a median titer (0.78 g/L) that was higher than those of the reference strain - trpD9923 (orange), and the in silico implementation of the two experimentally implemented strains, W3110 trpD9923/pJLaroGfbr (black) and W3110 trpD9923/pJLaroGfbrtktA (red). b Anthranilate curves of the designs for improving the performance of the in-silico implementation of W3110 trpD9923/pJLaroGfbrtktA - eK_trpD9923_d2. These designs attained a median titer of 1.16 g/L, superior to the simulated titers of the three experimental strains and the NOMAD designs from eK_trpD9923. Source data are provided as a Source Data file.
