Fig. 1: Surround suppression is stronger in PixON RGCs than in ON alpha RGCs.
a En-face view of a PixON (purple) and an ON alpha (brown) dendritic arbor (maximum intensity z-projection after manual tracing). b Average dendritic stratification of PixON (n = 19) and ON alpha (n = 10) RGCs within the inner nuclear layer (INL), inner plexiform layer (IPL), and ganglion cell layer (GCL). Dotted lines refer to the ON and OFF choline acetyltransferase (ChAT) bands used to determine stratification. Shaded region indicates standard error of the mean. c Example peristimulus time histograms recorded from a PixON RGC in response to preferred size and full-field light spot stimuli. The gray horizontal bar indicates the 1-second presentation of the 250 R*/rod/s spot stimulus from a background luminance of ~0.3 R*/rod/s. d Same as (c), but recorded from an ON alpha RGC. e Mean spike rates recorded from a PixON RGC (purple) and an ON alpha RGC (brown) in response to a range of spot sizes. Shaded region indicates the standard error of the mean. Arrows indicate the preferred spot size for each RGC. f Surround suppression of spiking response for PixON (n = 55) and ON alpha (n = 90) RGCs. Dots indicate data from individual cells. Bar plots indicate average ± s.e.m., *p < 0.05, two-sided Welch’s t test. g–j Same as (c–f) but measuring excitatory conductances via whole-cell voltage clamp configuration. j PixON (n = 37) and ON alpha (n = 31). k–n Same as (c–e) but measuring inhibitory conductances via whole-cell voltage clamp configuration. n PixON (n = 32) and ON alpha (n = 21). Source data are provided as a Source Data file.
