Fig. 6: Wide-field amacrine cell regulation near BC output synapses contributes to stronger surround suppression of PixON RGC excitatory responses.
a PixON excitatory conductances evoked before (top) and after (bottom) bath application of a glycine receptor antagonist (strychnine), a GABAA receptor antagonist (gabazine), a GABAB receptor antagonist (saclofen), a GABAC receptor antagonist (TPMPA), or NaV channel blocker (TTX). The gray horizontal bar indicates a 1-second presentation of the stimulus. Note: The response to full field stimuli in the TPMPA + TTX conditions was shifted down 2 nS to improve visibility. b Surround suppression of excitatory conductances in control and antagonists conditions. Dots indicate data from individual cells strychnine (n = 3), gabazine (n = 3), saclofen (n = 4), TPMPA (n = 6), TTX (n = 5), TPMPA + TTX (n = 5). Bar plots indicate average ± s.e.m., *p < 0.05, paired, two-sided, two-sample Student’s t test. c SBFSEM slice (top) and reconstruction (bottom) showing an AC neurite (cyan) forming an inhibitory synapse (yellow) onto a BC (gray), which then forms a ribbon synapse (red arrow) onto a PixON RGC dendrite (purple). d A zoomed-out En-face (top) and orthogonal (bottom) view of the AC from (c). e Reconstruction of nearest presynaptic ACs to T6 BC-to-PixON (left) and T6-to-ON alpha (right) ribbon synapses. f Distance to nearest inhibitory from T6 BC output synapses (PixON n = 51, ON alpha n = 26) and T7 BC output synapses (PixON n = 14, ON alpha n = 17). Dots indicate data from each BC-to-RGC synapse. Bar plots indicate average ± s.e.m., *p < 0.05, two-sided Welch’s t test. Source data are provided as a Source Data file. Data come from one reconstruction.
