Fig. 6: Osteoclast conditional knockout Gpr54 mice (Gpr54 cKO) and Kiss1 mice (Kiss1 cKO), whole-body knockout mice of Dusp18 (Dusp18−/−) mice exhibited bone loss and osteoclast hyperactivation. | Nature Communications

Fig. 6: Osteoclast conditional knockout Gpr54 mice (Gpr54 cKO) and Kiss1 mice (Kiss1 cKO), whole-body knockout mice of Dusp18 (Dusp18−/−) mice exhibited bone loss and osteoclast hyperactivation.

From: Kisspeptin-10 binding to Gpr54 in osteoclasts prevents bone loss by activating Dusp18-mediated dephosphorylation of Src

Fig. 6

a, c, e, g, i, k Representative micro-CT images of femoral trabecular bone and cortical bone. 4-month-old WT and Gpr54 cKO mice (n = 7 per group including 4 female and 3 male mice) (a, c), 4-month-old WT and Kiss1 cKO mice (n = 6 per group including 3 female and 3 male mice) (e, g) and 8-week-old WT and Dusp18−/− mice with or without treatment of KX2-391 or (DSS)*6-KP-10 (n = 7 for WT mice including 3 female and 4 male mice, n = 5 for each other group including 2 female and 3 male Dusp18−/− mice) (i, k). Scale bar, 500 µm. b, d, f, h, j, l Bone parameters analysis of femurs from the mice above. 2-month-old WT and Gpr54 cKO mice (b, d), 4-month-old WT and Kiss1 cKO mice (f, h), 4-month-old and Dusp18−/− mice (j, l). BMD bone mineral density, BV/TV bone volume as a fraction of total bone volume, Tb.Th trabecular thickness, Tb.N trabecular number, Tb.Sp trabecular separation. m, o, q Representative TRAP staining images of femurs from the mice above. 4-month-old and Gpr54 cKO mice (m), 4-month-old and Kiss1 cKO mice (o) and 4-month-old and Dusp18−/− mice (q). Scale bar, 200 µm. n, p, r Osteoclast parameters analysis of femurs from the mice above. 4-month-old WT and Gpr54 cKO mice (n), 4-month-old and Kiss1 cKO mice (p), 4-month-old and Dusp18−/− mice (r). Data represent means ± SEM. P values were determined by two-tailed Student’s t-test (b, d, f, h, n, p) or one-way ANOVA analysis (j, l, r). Representative results were obtained from at least three independent experiments. Source data are provided as a Source Data file.

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