Fig. 2: BARNI channel activation increases evoked seizure thresholds.

a Representative CA1 LFP responses to ipsilateral perforant pathway input electrical stimulation. Initial 1 s biphasic electrical stimulation (inset 1) of 80 µA failed to trigger a seizure, while subsequent 100 µA stimulation evoked sustained seizure activity with rhythmic bursting (inset 2). b Bradanicline elevated seizure thresholds (F(1, 8) = 8.96, P = 0.017), specifically related to an interaction for higher thresholds in BARNI animals (F(1, 8) = 9.48, P = 0.015, two-way RM ANOVA). c Seizure threshold shifts, normalized to the vehicle response of each mouse, were likewise elevated in BARNI vs. Scramble animals (t = 3.02, P = 0.017, two-sided t-test). d Evoked seizure durations did not vary between animals with bilateral hippocampal expression of BARNI or Scramble constructs (F(1, 8) = 1.67, P = 0.233), nor with bradanicline administration (F(1, 8) = 0.003, P = 0.958) or due to an interaction of those two factors (F(1, 8) = 0.046, P = 0.835, two-way RM ANOVA). e Seizure duration shifts, normalized to the vehicle response for each mouse, were similarly unchanged between vector groups (t = 0.34, P = 0.739, two-sided t-test). Bradanicline (100 mg/kg) was delivered i.p. 45 min prior to testing. In b and d, light lines show individual animal responses. Mean ± SEM, n = 5 mice per vector injection group. *P < 0.05. Multiple comparisons used two-sided Bonferroni correction. Source data are provided as a Source Data file.