Fig. 1: Improved resistance to ischemia-reperfusion injury (IRI) following carbohydrate loading with 50% sucrose water.

a Experimental group (left) and serum urea levels (right; AUC) post-renal IRI after one week of preconditioning with Ctrl or HC. Created with BioRender.com. b Serum creatinine levels at day 3 post-renal IRI. c. Representative cross sections of PAS-stained kidneys with necrotic area digitally highlighted in red (left; x10 magnification; scale bar 100 µm) and necrotic tissue area quantification (right) at day 2 post-renal IRI. d Relative Krt20 mRNA levels in kidney at day 2 post-renal IRI. e Experimental group (left) and serum urea levels (AUC) post-renal IRI after one week of preconditioning with Ctrl or HC. Created with BioRender.com. f Serum creatinine levels at day 2 post-renal IRI. g Representative cross sections of PAS-stained kidneys with necrotic area digitally highlighted in red (left; x10 magnification; scale bar 100 µm) and quantification of necrotic tissue area (right) at day 2 post-renal IRI. h Relative Krt20 mRNA levels in kidney at day 2 post-renal IRI. i Experimental group (left) and serum urea levels (AUC) post-renal IRI after one week of preconditioning with Ctrl or HC. Created with BioRender.com. j Representative cross sections of PAS-stained kidneys with necrotic area digitally highlighted in red (left; x10 magnification; scale bar 100 µm) and quantification of necrotic tissue area (right) at day 2 post-renal IRI. k Relative Krt20 mRNA levels in kidney at day 2 post-renal IRI. l Kaplan-Meier survival curve post-renal IRI after one week of preconditioning with Ctrl or HC. m Serum aspartate aminotransferase (AST) and (n) alanine aminotransferase (ALT) enzyme levels at the indicated time post-hepatic IRI. o Representative cross sections of H&E-stained livers (x20 magnification; scale bar 30 µm) at baseline or at day 1 pos-thepatic IRI. *p values for a-k were calculated with unpaired two-tailed T-test, and for l with two-way repeated measures (RM) ANOVA with Geisser-Greenhouse correction and n, m with two-way ANOVA followed by a Sidak’s post hoc analysis, *p < 0.05 **p < 0.01. p values for a = 0.303, for b = 0.0234, for c = 0.0154, for d = 0.0345, for e = 0.0352, for f = 0.0467, for g = 0.0246, for h = 0.0445, for i = 0.0107, for j =, for k = 0.0427, for l = 0.0462, for m = 0.0346, for n = 0.0252. Images 1a, 1e and 1i were partly created with BioRender.com. ♂ indicates males, ♀ indicates females;. In a-d and l-o, experiments were carried out in 10-weeks old male mice; in e-h, in 10-week-old female mice; in i-k, in 22-month-old male mice. Sample sizes: (a–c), n = 6-7, n = 7 for Ctrl diet, and n = 6 for HC diet; (d) n = 9 in all experimental groups; (e–h), n = 7 in all experimental groups; (i), n = 10 in all experimental groups (2 experiments pooled, experiment 1: n = 7 in all experimental groups, experiment 2: n = 3 in all experimental groups; (j-k) n = 7 in all experimental groups; (l–o), n = 6 for all conditions. Data shown as mean ± SD. See also Supplementary Data Fig. 1.