Fig. 3: Carbohydrate loading induces FGF21 expression.
From: Short-term hypercaloric carbohydrate loading increases surgical stress resilience by inducing FGF21
a Serum FGF21 levels after one week of preconditioning with control or low protein diet (LP) with control (Ctrl) or 50% sucrose-water (HC). b Relative liver Fgf21 mRNA levels after one week of preconditioning with the indicated diet. c Relative kidney Fgf21 mRNA levels after one week of preconditioning with the indicated diet. d Linear regression showing the relationship between serum FGF21 levels versus protein intake and (e) serum urea (AUC). R2 coefficient was calculated using Pearson’s method. f Serum FGF21 levels after one week of preconditioning with Ctrl, HC or HC with cysteine oral gavage (HC + Cysteine). g Volcano plot of liver differential expressed genes (DEG) with FDR < 0.05 and log2FC > 2 from FGF21 overexpressing male mice from GSE39313. h Fold change of top DEGs from GSE39313 in liver after one week of preconditioning with HC compared to Ctrl. *p values of a,b were calculated with two-way ANOVA followed by a Tukey’s post hoc analysis, d,e are linear regressions and r squared Pearson’s coefficient, f with one-way ANOVA and h with unpaired two-tailed T-test with Bonferroni correction, *p < 0.05 **p < 0.01 ***p < 0.001 ****p < 0.0001. p-values for a = 0.0001, for b = 0.0001, for d < 0.0001, for f < 0.0001, for h = 0.0003. In all panels, experiments were carried out in 10-weeks old male mice. Sample sizes: (a–c), n = 8; (d-e), n = 32; (f), n = 5 for all conditions; (h), n = 8 for all conditions. Data in all panels are shown as mean ± SD. See also Supplementary Data Figs. 4 and 5.
