Fig. 3: RBD-specific antibody responses to DNA-VLPs. | Nature Communications

Fig. 3: RBD-specific antibody responses to DNA-VLPs.

From: Enhancing antibody responses by multivalent antigen display on thymus-independent DNA origami scaffolds

Fig. 3

a Mice were sequentially immunized with monomeric RBD and DNA-VLPs of varying copy number. b RBD-specific IgG endpoint dilutions revealed enhanced antibody responses for DNA-VLP-30x compared to both monomeric RBD and DNA-VLP-6x. c Serum neutralization titers expressed as NT50 values against pseudoviruses modeling the ancestral Wuhan-1 strain. d Serum neutralization titers expressed as NT50 against native Wuhan-1 SARS-CoV-2. e IgM and IgG titers of RBD-specific antibodies elicited in Tcra–/– and wild-type mice after sequential immunization with DNA-VLP-30x. N = 5 female mice were used in each experimental group. Error bars represent the standard error of the mean. Non-responder mice (denoted as n.d. = not detectable) were not considered for statistical analysis. One-way ANOVA was performed followed by Dunnett’s T3 multiple comparison test at α = 0.05 for (b, c). Two-sided Welch’s t-test was performed at α = 0.05 for (d). For (b), Boost 1: p = 0.0021 for Monomer:DNA-VLP-30x, p = 0.0022 for DNA-VLP-6x:DNA-VLP-30x; Boost 2: p = 0.0048 for DNA-VLP-6x:DNA-VLP-30x. For (c), Boost 2: p = 0.0001 for Monomer:DNA-VLP-30x, p = 0.0001 for DNA-VLP-6x:DNA-VLP-30x; Boost 2 Normalized: p = 0.0005 for Monomer:DNA-VLP-30x; p = 0.0002 for DNA-VLP-6x:DNA-VLP-30x. *p < 0.05; **p < 0.01; ***p < 0.001.

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