Fig. 4: Scaffold-specific antibody responses to DNA-VLPs and P-VLPs. | Nature Communications

Fig. 4: Scaffold-specific antibody responses to DNA-VLPs and P-VLPs.

From: Enhancing antibody responses by multivalent antigen display on thymus-independent DNA origami scaffolds

Fig. 4

a Mice were sequentially immunized with monomeric RBD, DNA-VLP-30x, or ferritin-based P-VLP-24x. b RBD-specific and scaffold-specific IgG endpoint dilutions for the P-VLP immunization. c RBD-specific and scaffold-specific IgG endpoint dilutions for the DNA-VLP immunization and dilution curves for Boost 2 of the DNA-specific IgG ELISA. The DNA-specific IgG control was diluted from 10 µg/ml. N = 5 male mice were used in each experimental group. Error bars represent the standard error of the mean. Non-responder mice (denoted as n.d. = not detectable) were not considered for statistical analysis. One-way ANOVA was performed followed by Dunnett’s T3 multiple comparison test at α = 0.05 for (b, c). For (b), RBD-specific IgG titers: p = 0.0199 for Prime:Boost 1, p = 0.0209 for Boost1:Boost 2; Ferritin-specific IgG titers: p = 0.0036 for Prime:Boost 1, p = 0.0291 for Prime:Boost 2. For (c), p = 0.0094 for Prime:Boost 1, p = 0.0127 for Prime:Boost 2. *p < 0.05; **p < 0.01; ***p < 0.001.

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