Fig. 5: DREADDs effectively silence dentate gyrus interneurons.
From: Subfield-specific interneuron circuits govern the hippocampal response to novelty in male mice
a Post-hoc confocal fluorescence image of a brain slice from a PV-Cre animal injected with floxed h4MDi (red) and non-floxed GCaMP6f (green; Methods) together with PV immunolabelling (yellow). Section is representative for n = 6 sections obtained from N = 3 animals. b Same as in a but for a SOM-Cre animals. Section is representative for n = 6 sections obtained from N = 3 animals. c Fraction of DG PV-INs (green) and DG SOM-INs (magenta) identified by immunolabelling that were co-expressing h4MDi after injection of the double-floxed virus in the respective Cre-animals (Methods). d Activity of PV- and SOM-INs before (left half) and after (right half) application of clozapine in PV- and SOM-Cre mice transfected with h4MDi, respectively. Lines indicate data from individual cells. All statistical tests are two-sided. For exact p values see Supplementary Table 2.
